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Low-dose rapamycin enhanced the effects of iMSC 2B-Fc/Luci to reduce primary HO. (A) Schematic view of the experiments. Rapamycin (0.3 mg/kg, 5 times/wk) was intraperitoneally administrated after CTX injected on day 7. Cells in 100 μL aMEM (iMSC EiP/Luc or iMSC 2B-Fc/Luc , 1.5 × 10 6 ) were locally administered every 7 d from day 8. (B) The body weight of mice was monitored every 3-5 d. (C) Representative X-ray and μCT images from each group. Arrowheads indicate HO. Scale bar, 10 mm. (D) Average of HO volume on day 28. (E) BMC of HO lesions. (F) The exercise capacities were analyzed using the rotarod test. (G) Endurance tests were evaluated using a rodent treadmill. (H, I) Activin-A and Fc-fragment in mouse serum were detected <t>by</t> <t>ELISA.</t> Results represent the mean ± SEM. * , p < .05; ** , p < .01; *** , p < .001. Data were analyzed using one-way ANOVA with Dunnett’s multiple comparison test. Data in (I) were analyzed using one-way ANOVA with Tukey’s multiple comparison test. Abbreviations: HO, heterotopic ossification; iMSC, mesenchymal stem/stromal cells derived from induced pluripotent stem cells; CTX, cardiotoxin; aMEM, Alpha Modification of Eagle's Medium; BMC, bone mineral content.
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Low-dose rapamycin enhanced the effects of iMSC 2B-Fc/Luci to reduce primary HO. (A) Schematic view of the experiments. Rapamycin (0.3 mg/kg, 5 times/wk) was intraperitoneally administrated after CTX injected on day 7. Cells in 100 μL aMEM (iMSC EiP/Luc or iMSC 2B-Fc/Luc , 1.5 × 10 6 ) were locally administered every 7 d from day 8. (B) The body weight of mice was monitored every 3-5 d. (C) Representative X-ray and μCT images from each group. Arrowheads indicate HO. Scale bar, 10 mm. (D) Average of HO volume on day 28. (E) BMC of HO lesions. (F) The exercise capacities were analyzed using the rotarod test. (G) Endurance tests were evaluated using a rodent treadmill. (H, I) Activin-A and Fc-fragment in mouse serum were detected <t>by</t> <t>ELISA.</t> Results represent the mean ± SEM. * , p < .05; ** , p < .01; *** , p < .001. Data were analyzed using one-way ANOVA with Dunnett’s multiple comparison test. Data in (I) were analyzed using one-way ANOVA with Tukey’s multiple comparison test. Abbreviations: HO, heterotopic ossification; iMSC, mesenchymal stem/stromal cells derived from induced pluripotent stem cells; CTX, cardiotoxin; aMEM, Alpha Modification of Eagle's Medium; BMC, bone mineral content.
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Low-dose rapamycin enhanced the effects of iMSC 2B-Fc/Luci to reduce primary HO. (A) Schematic view of the experiments. Rapamycin (0.3 mg/kg, 5 times/wk) was intraperitoneally administrated after CTX injected on day 7. Cells in 100 μL aMEM (iMSC EiP/Luc or iMSC 2B-Fc/Luc , 1.5 × 10 6 ) were locally administered every 7 d from day 8. (B) The body weight of mice was monitored every 3-5 d. (C) Representative X-ray and μCT images from each group. Arrowheads indicate HO. Scale bar, 10 mm. (D) Average of HO volume on day 28. (E) BMC of HO lesions. (F) The exercise capacities were analyzed using the rotarod test. (G) Endurance tests were evaluated using a rodent treadmill. (H, I) Activin-A and Fc-fragment in mouse serum were detected <t>by</t> <t>ELISA.</t> Results represent the mean ± SEM. * , p < .05; ** , p < .01; *** , p < .001. Data were analyzed using one-way ANOVA with Dunnett’s multiple comparison test. Data in (I) were analyzed using one-way ANOVA with Tukey’s multiple comparison test. Abbreviations: HO, heterotopic ossification; iMSC, mesenchymal stem/stromal cells derived from induced pluripotent stem cells; CTX, cardiotoxin; aMEM, Alpha Modification of Eagle's Medium; BMC, bone mineral content.
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Low-dose rapamycin enhanced the effects of iMSC 2B-Fc/Luci to reduce primary HO. (A) Schematic view of the experiments. Rapamycin (0.3 mg/kg, 5 times/wk) was intraperitoneally administrated after CTX injected on day 7. Cells in 100 μL aMEM (iMSC EiP/Luc or iMSC 2B-Fc/Luc , 1.5 × 10 6 ) were locally administered every 7 d from day 8. (B) The body weight of mice was monitored every 3-5 d. (C) Representative X-ray and μCT images from each group. Arrowheads indicate HO. Scale bar, 10 mm. (D) Average of HO volume on day 28. (E) BMC of HO lesions. (F) The exercise capacities were analyzed using the rotarod test. (G) Endurance tests were evaluated using a rodent treadmill. (H, I) Activin-A and Fc-fragment in mouse serum were detected <t>by</t> <t>ELISA.</t> Results represent the mean ± SEM. * , p < .05; ** , p < .01; *** , p < .001. Data were analyzed using one-way ANOVA with Dunnett’s multiple comparison test. Data in (I) were analyzed using one-way ANOVA with Tukey’s multiple comparison test. Abbreviations: HO, heterotopic ossification; iMSC, mesenchymal stem/stromal cells derived from induced pluripotent stem cells; CTX, cardiotoxin; aMEM, Alpha Modification of Eagle's Medium; BMC, bone mineral content.
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Image Search Results


Low-dose rapamycin enhanced the effects of iMSC 2B-Fc/Luci to reduce primary HO. (A) Schematic view of the experiments. Rapamycin (0.3 mg/kg, 5 times/wk) was intraperitoneally administrated after CTX injected on day 7. Cells in 100 μL aMEM (iMSC EiP/Luc or iMSC 2B-Fc/Luc , 1.5 × 10 6 ) were locally administered every 7 d from day 8. (B) The body weight of mice was monitored every 3-5 d. (C) Representative X-ray and μCT images from each group. Arrowheads indicate HO. Scale bar, 10 mm. (D) Average of HO volume on day 28. (E) BMC of HO lesions. (F) The exercise capacities were analyzed using the rotarod test. (G) Endurance tests were evaluated using a rodent treadmill. (H, I) Activin-A and Fc-fragment in mouse serum were detected by ELISA. Results represent the mean ± SEM. * , p < .05; ** , p < .01; *** , p < .001. Data were analyzed using one-way ANOVA with Dunnett’s multiple comparison test. Data in (I) were analyzed using one-way ANOVA with Tukey’s multiple comparison test. Abbreviations: HO, heterotopic ossification; iMSC, mesenchymal stem/stromal cells derived from induced pluripotent stem cells; CTX, cardiotoxin; aMEM, Alpha Modification of Eagle's Medium; BMC, bone mineral content.

Journal: JBMR Plus

Article Title: Combined rapamycin and mesenchymal stem/stromal cells derived from induced pluripotent stem cells-mediated delivery of ACVR2B-Fc fusion protein reduces heterotopic ossification in a mouse model of fibrodysplasia ossificans progressiva

doi: 10.1093/jbmrpl/ziaf068

Figure Lengend Snippet: Low-dose rapamycin enhanced the effects of iMSC 2B-Fc/Luci to reduce primary HO. (A) Schematic view of the experiments. Rapamycin (0.3 mg/kg, 5 times/wk) was intraperitoneally administrated after CTX injected on day 7. Cells in 100 μL aMEM (iMSC EiP/Luc or iMSC 2B-Fc/Luc , 1.5 × 10 6 ) were locally administered every 7 d from day 8. (B) The body weight of mice was monitored every 3-5 d. (C) Representative X-ray and μCT images from each group. Arrowheads indicate HO. Scale bar, 10 mm. (D) Average of HO volume on day 28. (E) BMC of HO lesions. (F) The exercise capacities were analyzed using the rotarod test. (G) Endurance tests were evaluated using a rodent treadmill. (H, I) Activin-A and Fc-fragment in mouse serum were detected by ELISA. Results represent the mean ± SEM. * , p < .05; ** , p < .01; *** , p < .001. Data were analyzed using one-way ANOVA with Dunnett’s multiple comparison test. Data in (I) were analyzed using one-way ANOVA with Tukey’s multiple comparison test. Abbreviations: HO, heterotopic ossification; iMSC, mesenchymal stem/stromal cells derived from induced pluripotent stem cells; CTX, cardiotoxin; aMEM, Alpha Modification of Eagle's Medium; BMC, bone mineral content.

Article Snippet: The serum was harvested after centrifugation for 15 min at 2000 × g . Activin-A and Fc fragment in serum were detected using Human/Mouse/Rat Activin-A Quantikine ELISA Kit (DAC00B, R&D Systems) and Human Fcγ (Fc Fragment of IgG) ELISA Kit (E-EL-H1745, Elabscience).

Techniques: Injection, Enzyme-linked Immunosorbent Assay, Comparison, Derivative Assay, Modification

Low-dose rapamycin enhanced the effects of iMSC 2B-Fc/Luci to reduce recurrent HO. (A) Schematic view of the experiments. Rapamycin (0.3 mg/kg, 5 times/wk) was intraperitoneally administrated after HO was surgically removed on day 21. Cells in 100 μL aMEM (iMSC EiP/Luc or iMSC 2B-Fc/Luc , 1.5 × 10 6 ) were locally administered every 7 d from day 22. (B) The body weight of mice was monitored every 3-5 d. (C) Representative X-ray and μCT images from each group. Arrowheads indicate primary HO, and arrows indicate recurrent HO. Scale bar, 10 mm. (D) Average of HO volume on day 21 (pre- and post-surgery) and recurrent HO (volume day35 – volume post-surgery ). (E) BMC of HO lesions. (F, G) The exercise capacity and endurance were evaluated using the rodent rotarod and treadmill tests, respectively. (H, I) Activin-A and Fc-fragment in mouse serum were detected by ELISA. Results represent the mean ± SEM. ** , p < .01; *** , p < .001. Data were analyzed using one-way ANOVA with Dunnett’s multiple comparison test. Data in (D) were analyzed using two-way ANOVA with Tukey’s multiple comparison test. Data in (I) were analyzed using one-way ANOVA with Tukey’s multiple comparison test. Abbreviations: HO, heterotopic ossification; iMSC, mesenchymal stem/stromal cells derived from induced pluripotent stem cells.

Journal: JBMR Plus

Article Title: Combined rapamycin and mesenchymal stem/stromal cells derived from induced pluripotent stem cells-mediated delivery of ACVR2B-Fc fusion protein reduces heterotopic ossification in a mouse model of fibrodysplasia ossificans progressiva

doi: 10.1093/jbmrpl/ziaf068

Figure Lengend Snippet: Low-dose rapamycin enhanced the effects of iMSC 2B-Fc/Luci to reduce recurrent HO. (A) Schematic view of the experiments. Rapamycin (0.3 mg/kg, 5 times/wk) was intraperitoneally administrated after HO was surgically removed on day 21. Cells in 100 μL aMEM (iMSC EiP/Luc or iMSC 2B-Fc/Luc , 1.5 × 10 6 ) were locally administered every 7 d from day 22. (B) The body weight of mice was monitored every 3-5 d. (C) Representative X-ray and μCT images from each group. Arrowheads indicate primary HO, and arrows indicate recurrent HO. Scale bar, 10 mm. (D) Average of HO volume on day 21 (pre- and post-surgery) and recurrent HO (volume day35 – volume post-surgery ). (E) BMC of HO lesions. (F, G) The exercise capacity and endurance were evaluated using the rodent rotarod and treadmill tests, respectively. (H, I) Activin-A and Fc-fragment in mouse serum were detected by ELISA. Results represent the mean ± SEM. ** , p < .01; *** , p < .001. Data were analyzed using one-way ANOVA with Dunnett’s multiple comparison test. Data in (D) were analyzed using two-way ANOVA with Tukey’s multiple comparison test. Data in (I) were analyzed using one-way ANOVA with Tukey’s multiple comparison test. Abbreviations: HO, heterotopic ossification; iMSC, mesenchymal stem/stromal cells derived from induced pluripotent stem cells.

Article Snippet: The serum was harvested after centrifugation for 15 min at 2000 × g . Activin-A and Fc fragment in serum were detected using Human/Mouse/Rat Activin-A Quantikine ELISA Kit (DAC00B, R&D Systems) and Human Fcγ (Fc Fragment of IgG) ELISA Kit (E-EL-H1745, Elabscience).

Techniques: Enzyme-linked Immunosorbent Assay, Comparison, Derivative Assay